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目的研究热化联合对肺部肿瘤细胞生长的影响及其可能的机制。方法参考临床常用剂量,采用43℃加热联合120μg/L紫杉醇(热化联合组)、43℃加热联合120μg/L紫杉醇并加入30μmol/L活性氧(ROS)特异抑制剂NAC(NAC组)、单纯使用120μg/L紫杉醇(单纯化疗组)处理H446细胞,以未处理的H446细胞作对照,应用流式细胞术(FCM)检测细胞凋亡率,荧光法检测细胞内活性氧(ROS),蛋白免疫印记法(Western blotting)检测Caspase-3的表达,SPSS 13.0对数据进行统计分析。结果热化联合组细胞凋亡率高于其余各组(P<0.05);ROS在热化联合组增高(P<0.05),NAC可抑制其表达;Caspase-3表达在热化联合组中表达明显增高(P<0.05),但可被NAC抑制(P<0.05)。结论热化联合应用可以明显诱导H446细胞发生凋亡,可能是通过诱导ROS的产生,通过caspase途径实现的。
Objective To study the effect of thermo-chemical combination on lung tumor cell growth and its possible mechanism. Methods According to the commonly used clinical dose, heat shock combined with 120μg / L paclitaxel at 43 ℃, combined with 120μg / L paclitaxel at 43 ℃ and NAC (NAC group) with 30μmol / L reactive oxygen species (ROS) H446 cells were treated with 120μg / L paclitaxel (chemotherapy alone group), and untreated H446 cells were used as control. Flow cytometry (FCM) was used to detect the apoptosis rate. Fluorescence was used to detect intracellular reactive oxygen species (ROS) Western blotting was used to detect the expression of Caspase-3, and SPSS 13.0 was used for statistical analysis. Results The apoptotic rate of the combined heat-treated group was higher than that of the other groups (P <0.05). The ROS level was increased in the heat-combined group (P <0.05), and NAC inhibited the expression of Caspase-3 protein (P <0.05), but could be inhibited by NAC (P <0.05). Conclusion Combined application of heating can obviously induce the apoptosis of H446 cells, which may be through the caspase pathway by inducing the production of ROS.