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目的比较浓集菌涂片抗酸染色法(涂片法)、实时荧光定量聚合酶链反应(FQ-PCR)法、结核培养(L-J培养)和血清学方法检测血清结核抗体(TB-Ab)对临床结核诊断的应用价值。方法用涂片法,FQ-PCR法和L-J培养,对200份疑似结核病人呼吸道标本进行检测,同时检测其TB-Ab。结果200份标本,总阳性率涂片法为20.5%,FQ-PCR法为32.5%,培养法为25.0%,血清结核抗体为42.5%;四种方法的灵敏度、特异度分别为:涂片法36.8%和94.3%,FQ-PCR法64.2%和96.2%,培养敏感度为50.5%,特异度为98.1%,血清结核抗体63.2%和76.2%。结论结核培养作为结核诊断的金标准仍是不可缺少的方法,但周期长;FQ-PCR法快速特异性高,有较好诊断价值;血清学方法检测血清结核抗体方便,简单;涂片法直观、简便、价廉,仍将是结核病实验诊断的重要手段,同时检测可从不同方面满足临床需要。
OBJECTIVE To compare the antitumor activity of TB-Ab with smear method, FQ-PCR method, tuberculosis culture (LJ culture) and serological methods, The clinical value of the diagnosis of tuberculosis. Methods The smear method, FQ-PCR method and L-J culture were used to detect 200 TB samples of suspected TB patients and detect their TB-Ab. Results The total positive rate was 20.5% in smear method, 32.5% in FQ-PCR method, 25.0% in culture method and 42.5% in tuberculosis antibody. The sensitivities and specificities of the four methods were as follows: smear method 36.8% and 94.3% respectively. The FQ-PCR was 64.2% and 96.2% respectively. The culture sensitivity was 50.5%, the specificity was 98.1%, and the serum TB antibody was 63.2% and 76.2% respectively. Conclusion Tuberculosis is an indispensable method for gold standard of tuberculosis diagnosis, but it has a long cycle. FQ-PCR method is rapid and specific, and it has a good diagnostic value. Serological methods for detecting serum TB antibody are convenient and simple, smear method is intuitive , Simple, cheap, will still be an important tool for the experimental diagnosis of tuberculosis, while testing can meet the clinical needs in different aspects.