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目的研究单核细胞对异体血管内皮细胞(VEC)的活化作用以及产生干扰素诱导蛋白10(IP-10)、干扰素诱导的T细胞α型趋化因子(I-TAC)的效果。方法人外周血中分离单核细胞,酶消化法分离人主动脉内皮细胞,建立单核细胞与VEC共培养系统。用流式细胞仪(FACS)检测VEC表面粘附分子CD54、CD62E表达的情况,用RT-PCR检测VEC内I-TAC和IP-10mRNA的表达情况。结果 RT-PCR检测结果表明,VEC与同种异体单核细胞共培养24h后,细胞内IP-10和I-TACmRNA表达水平显著增加(P<0.05),且在48、72h的表达维持在较高的水平。FACS检测结果表明,正常培养的VEC少量表达CD54分子,不表达CD62E分子。与同种异体单核细胞共培养24h后,VEC表面CD62E和CD54的表达水平明显上调(P<0.05)。结论在同种异体单核细胞-血管内皮细胞免疫反应中,单核细胞能活化血管内皮细胞,使内皮细胞表达IP-10、I-TAC等趋化因子和CD54、CD62E等粘附分子,参与对移植器官的排斥反应。
Objective To investigate the effects of monocytes on the activation of allogenic vascular endothelial cells (VECs) and on the production of interferon-inducible protein 10 (IP-10) and interferon-induced T-cell α-chemokine (I- TAC). Methods Human peripheral blood mononuclear cells were isolated by enzyme digestion of human aortic endothelial cells, monocytes and VEC co-culture system established. Flow cytometry (FACS) was used to detect the expression of adhesion molecules CD54 and CD62E on VECs. The expression of I-TAC and IP-10 mRNA in VECs was detected by RT-PCR. Results The results of RT-PCR showed that the expression of IP-10 and I-TAC mRNA in VEC and allogeneic monocytes were significantly increased (P <0.05) at 24 and 72 h High level. FACS test results showed that the normal culture of VEC small expression of CD54 molecules, do not express CD62E molecules. After co-cultured with allogeneic monocytes for 24 hours, the expressions of CD62E and CD54 on VEC surface were significantly up-regulated (P <0.05). Conclusion Monocytes can activate vascular endothelial cells in the allo-monocyte-vascular endothelial cell immune response and induce endothelial cells to express chemokines such as IP-10 and I-TAC and adhesion molecules such as CD54 and CD62E, Rejection to transplanted organs.