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目的探讨HLA-G基因单核苷酸多态性(SNP)与广西儿童支气管哮喘(哮喘)发病的关联性。方法对广西哮喘患儿88例(哮喘组)及112例健康对照儿童(健康对照组)采用基于基质辅助激光解吸电离-飞行时间质谱技术检测了HLA-G基因SNP的分型变化(SNP位点包括rs1736936、rs3115630、rs1632947、rs1233334、rs1736933、rs1736932、rs1631950、rs1704和rs1063320),探讨HLA-G基因SNP与哮喘发生的关联。采用SPSS 13.0软件进行分析。结果发现rs1063320、rs1632947共2个位点的基因型构成比在哮喘组与对照组有显著性差异(P<0.05);rs1631950、rs1736932、rs1736933、rs1736936共4个位点的基因型在哮喘组和健康对照组有临界性差异(P=0.05～0.06)。其中rs1063320位点的CC基因型携带者与GG基因型携带者相比,哮喘危险性增加了1.27倍(P<0.05)。rs1632947位点的TT基因型携带者与CC基因型携带者相比,哮喘危险性增加了1.59倍(P=0.02)。结论 HLA-G基因SNP与广西儿童哮喘的发病相关。rs1063320位点的CC基因型和rs1632947位点的TT基因型是儿童哮喘的易感基因型。
Objective To investigate the association between single nucleotide polymorphism (SNP) of HLA-G gene and bronchial asthma (asthma) in Guangxi children. Methods The genotypes of SNPs in HLA-G gene were detected in 88 children with asthma (asthma group) and 112 healthy control children (healthy control group) by using matrix assisted laser desorption ionization-time of flight mass spectrometry (SNP) Including rs1736936, rs3115630, rs1632947, rs1233334, rs1736933, rs1736932, rs1631950, rs1704 and rs1063320, to explore the association between HLA-G SNPs and asthma. Using SPSS 13.0 software for analysis. The results showed that the genotypes of rs1063320 and rs1632947 in two loci were significantly different from those in the asthma group and the control group (P <0.05). The genotypes of rs1631950, rs1736932, rs1736933 and rs1736936 in four groups were significantly lower in asthma group and There was a critical difference in healthy control group (P = 0.05 ~ 0.06). The rs1063320 locus CC genotype carriers compared with GG genotypes, asthma risk increased by 1.27 times (P <0.05). TT genotype rs1632947 locus carriers compared with CC genotype, asthma risk increased 1.59-fold (P = 0.02). Conclusion The SNP of HLA-G gene is associated with asthma in Guangxi children. The CC genotype at rs1063320 and the TT genotype at rs1632947 are susceptible genotypes in childhood asthma.