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用TPA及正丁酸钠诱导Raji细胞可生成EB病毒多聚酶(EBV—DP),诱导生成是在早期。此酶可被KCl及(NH_4)_2SO_4激活,鼻咽癌血清可中和此酶活性。用三种柱层析纯化的EBV—DP免疫BALB/c小鼠,通过杂交瘤技术筛得两株抗EBV—DP的单克隆杂交瘤细胞(3F1,6F2)。EBV—DP不单被EBV—DP单抗结合及中和其活性,也可被Biotech公司的EA—D单抗结合及中和。纯化的EBV—DP进行SDS凝胶电泳转渍后,以EBV—DP单抗进行免疫酶联染色,可显出EBV—DP二条多肽,分子量分别为49K及55K。如以Biotech的抗EA—D单抗代替EBV—DP单抗,用酶联染色同样可检出EBV—DP有49K及55K两条多肽,这与文献用Biotech公司的抗EA—D单抗检出的EA—D分子量49K及55K相符合。本文对Raji细胞诱导生成EBV—DP是EA抗原复合物成份之一(或正是EA—D)的可能性进行了讨论。
Raji cells induced by TPA and sodium butyrate can generate Epstein-Barr virus polymerase (EBV-DP), which is induced early. This enzyme can be activated by KCl and (NH_4)_2SO_4, and nasopharyngeal carcinoma serum can neutralize this enzyme activity. BALB/c mice were immunized with three column chromatography purified EBV-DP, and two hybridoma cells (3F1, 6F2) against EBV-DP were screened by hybridoma technique. EBV-DP is not only bound by EBV-DP mAb and neutralizes its activity, but it can also be combined and neutralized by Biotech’s EA-D monoclonal antibody. After purified EBV-DP was transfected by SDS gel electrophoresis, EBV-DP monoclonal antibody was used to perform immunoenzymatic staining, and two polypeptides of EBV-DP were revealed with molecular weights of 49K and 55K, respectively. If Biotech’s anti-EA-D monoclonal antibody is used instead of EBV-DP monoclonal antibody, EBV-DP has 49K and 55K polypeptides detected by enzyme-linked staining. This is in line with Biotech’s anti-EA-D monoclonal antibody. The EA-D has a molecular weight of 49K and 55K. This article discusses the possibility that Raji cells induce EBV-DP to be one of the components of the EA antigen complex (or exactly EA-D).