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目的采用超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS)对不同产地虫草化学成分进行鉴定与比较分析。方法采用超高效液相-飞行时间质谱(UPLC-Q-TOF-MS)及多元统计分析技术对实验结果进行分析,并对差异化合物进行鉴定。结果对不同产地的虫草进行了成分鉴定,核苷类物质含量高。不同产地虫草药材在组分种类和相对量上存在着一定的差异。湖南、湖北、丽江的虫草含有其他成分,丽江虫草含有大量的虫草素,湖南虫草含有一些黄酮类物质。采用主成分分析(PCA)将不同产地药材分别聚成不同组别,其中西藏(那曲)、四川、青海之间聚集紧密,而凉山、湖北、湖南及丽江虫草散布在四周,差别非常显著。结论 UPLC-Q-TOF-MS结合多元统计分析方法可为虫草药材的质量评价提供依据。
OBJECTIVE To identify and compare the chemical constituents of Cordyceps from different habitats by ultra performance liquid chromatography-time of flight mass spectrometry (UPLC-Q-TOF-MS). Methods The experimental results were analyzed by UPLC-Q-TOF-MS and multivariate statistical analysis techniques, and the compounds were identified. Results Cordyceps sinensis from different areas were identified, with high nucleoside content. Cordyceps herbs from different areas in the species composition and relative amount there is a certain difference. Cordyceps in Hunan, Hubei and Lijiang contain other ingredients. Lijiang Cordyceps contains a large amount of cordycepin, and Hunan Cordyceps contains some flavonoids. The principal component analysis (PCA) was used to separate the medicinal plants of different origins into different groups. Among them, Tibet (Naqu), Sichuan and Qinghai gathered together closely, while Cordyceps Liangshan, Hubei, Hunan and Lijiang were scattered around. The differences were significant. Conclusion UPLC-Q-TOF-MS combined with multivariate statistical analysis can provide the basis for the quality evaluation of Cordyceps sinensis.