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目的研究铁皮石斛的化学成分。方法采用硅胶色谱法、HP2MGL型大孔树脂色谱法、Sephadex LH-20、SephadexG-25凝胶色谱法、RP-HPLC制备柱色谱法进行分离纯化,通过现代波谱技术进行结构鉴定。结果鉴定了10个化合物,分别为2-甲氧基苯基-1-O-β-D-芹糖-(1→2)-β-D-葡萄糖苷(1),3,4,5-三甲氧基苯基-1-O-β-D-芹糖-(1→2)-β-D-葡萄糖苷(2),leo-nuriside A(3),(1′R)-1′-(4-羟基-3,5-二甲氧基苯基)-1-丙醇-4-O-β-D-葡萄糖苷(4),左旋丁香脂素(5),丁香脂素-4,4′-O-双-β-D-葡萄糖苷(6),(+)-丁香脂素-4-O-β-D-吡喃葡萄糖苷(7),icariol A2-4-O-β-D-glucopyranoside(8),(+)-lyoniresinol-3a-O-β-D-glu-copyranoside(9),裂异落叶松脂醇(10)。结论 1为新化合物,2～4,6～10为首次从本属植物中发现,5为首次从本植物中发现。
Objective To study the chemical composition of Dendrobium candidum. Methods Silica gel chromatography, HP2MGL macroporous resin chromatography, Sephadex LH-20, Sephadex G-25 gel chromatography and RP-HPLC preparative column chromatography were used for purification. The structure was identified by modern spectroscopic techniques. As a result, 10 compounds were identified as 2-methoxyphenyl-1-O-β-D-glucans- (1 → 2) -β-D-glucoside (1) (1 → 2) -β-D-glucoside (2), leo-nuriside A (3), (1’R) -1’- (4), levosuberosin (5), syringaresin-4, l- (4-hydroxy-3,5-dimethoxyphenyl) 4-O-bis-β-D-glucoside (6), (+) - syringaresin-4-O-β-D-glucopyranoside (7) D-glucopyranoside (8), (+) - lyoniresinol-3a-O-β-D-glu-copyranoside (9) Conclusion 1 is a new compound, 2 ~ 4, 6 ~ 10 for the first time from this genus found, 5 for the first time found from this plant.