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为观察维甲酸对生长期大鼠骺板软骨的损伤作用及谷康泰灵对此损伤的治疗效果 ,将 SD大鼠 44只随机分为正常组 (13只 )和维甲酸组 (31只 )。正常组给蒸馏水 ,维甲酸组每天以维甲酸 80 m g· kg- 1 灌胃。 15天后 2组各处死 5只 ,正常组 (余 8只 )继续观察 ,维甲酸组再随机分为无措施对照组 (8只 ,给等量蒸馏水 )、谷康泰灵治疗组 (10只 ,每天以 0 .0 8mg· kg- 1 谷康泰灵注射液腹腔注射 )和雌二醇治疗组 (8只 ,每周 3次 ,每次以 0 .0 5 mg苯甲酸雌二醇腹腔注射 ) ,疗程 30天。取大鼠股骨下端观察骺板软骨形态学变化。结果示维甲酸灌胃 15天后 ,大鼠骺板软骨宽度变窄 ,软骨细胞数量显著减少 ,软骨各层均有大片细胞坏死 ,以静止层和增殖层明显 ;肥大软骨细胞不能正常参与钙化 ,初级骨小梁血管进入骺板软骨受阻 ,初级骨小梁数量减少。谷康泰灵和雌激素治疗 30天后 ,损伤骺板软骨恢复明显 ,宽度增加 ,软骨细胞数量增多 ,肥大软骨细胞参与初级骨小梁形成正常。谷康泰灵的治疗效果好于雌激素。结论 :维甲酸对生长期大鼠骺板软骨有显著的损伤作用 ,这可能是其诱导骨质疏松的一个重要机理。动物长干骨中提取的多肽类物质谷康泰灵对维甲酸引起的大鼠骺板软骨损伤有明显的治疗作用。
In order to observe the effect of retinoic acid on the growth plateau cartilage injury in rats and the therapeutic effect of Gu Kang Tailing on this injury, 44 SD rats were randomly divided into normal group (13) and retinoic acid group (31). Distilled water was given to the normal group, and the retinoid group was intragastrically administered with Tretinoin 80 μg·kg-1 per day. After 15 days, 5 rats in each group were sacrificed. The normal group (more than 8) continued to observe, and the retinoid group was randomly divided into no-treatment control group (8 rats given equal distilled water) and Gu Kangtailing treatment group (10 rats daily). Intraperitoneal injection of 0.0 mg/kg G-Glutallin (0.20 mg·kg-1) and estradiol (8 mice, 3 times per week, 0.1 mg of estradiol benzoate intraperitoneally) 30 days. The rat femur was taken to observe the morphological changes of the chondral cartilage. The results showed that after intragastric administration of retinoic acid for 15 days, the rat chondral cartilage width became narrower and the number of chondrocytes was significantly reduced. There was a large number of necrotic cells in all layers of the cartilage, which was evident in the quiescent layer and the proliferative layer; hypertrophic chondrocytes could not normally participate in calcification. The trabecular blood vessels enter the tarsal articular cartilage and the number of primary trabecular bones is reduced. After 30 days of GuKang Tailing and estrogen treatment, the cartilage of the injured plate recovered significantly, the width increased, the number of chondrocytes increased, and the hypertrophic chondrocytes involved in primary trabecular bone formation. Gu Kang Tailing better than estrogen treatment. CONCLUSION: Retinoic acid has a significant damage effect on rat tarsal cartilage in growing rats, which may be an important mechanism for osteoporosis induction. GuTangling, a polypeptide extracted from the long bone of animal, has obvious therapeutic effect on retinoic acid-induced rat meibomian cartilage injury.