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以黑龙江13个育种单位6个积温带的83份大豆品种为材料,选择分布在大豆基因组19个连锁群的43对SSR引物进行检测,共检测出等位变异157个,每个引物检测到的等位变异数变化范围为2～7个,平均为3.65个。将聚丙烯酰胺凝胶电泳得到的谱带统计结果根据等位变异的片段大小数字化,用自行编制的ID Analysis 1.0软件进行数据分析。结果表明,仅需9对引物(Satt100、Sat_218、Satt514、Satt551、Satt380、Satt193、Satt191、Satt442、Sat_084)可将83份参试大豆品种完全区分开。构建了一套黑龙江省大豆品种的分子ID。
In this study, 83 SSR primers distributed in 19 linkage groups of soybean genome were tested with 83 soybean cultivars with 6 accumulated temperate zones in 13 breeding units in Heilongjiang Province. A total of 157 SSR primers were detected, of which alleles were detected by each primer The number of alleles varied from 2 to 7 with an average of 3.65. The band statistical results obtained by polyacrylamide gel electrophoresis were digitized according to the size of the allelic variant and analyzed by ID Analysis 1.0 software. The results showed that only 83 primer pairs (Satt100, Sat_218, Satt514, Satt551, Satt380, Satt193, Satt191, Satt442, Sat_084) could completely distinguish 83 tested soybean varieties. Constructed a set of molecular ID of soybean varieties in Heilongjiang Province.