论文部分内容阅读
探讨熊去氧胆酸(UDCA)和“一贯煎”在原发性胆汁性肝硬化小鼠中的疗效。对polyI∶C注射的C57BL/6雌性小鼠分别给予UDCA、“一贯煎”和两种药物联合治疗,分析不同药物治疗组血清ALP、-γGT的变化,实时荧光定量RT-PCR方法检测脾脏单个核细胞BCMA mRNA的表达,并分析肝门管区单个核细胞浸润率。结果表明,PBC模型鼠肝门管区单个核细胞浸润率、脾脏单个核细胞BCMA mRNA的表达较正常小鼠明显增加(P<0.05);不同治疗组较模型对照组ALP、-γGT显著下降(P<0.05),“一贯煎”或联合治疗还能减少肝门管区单个核细胞浸润率、下调脾脏单个核细胞BCMAmRNA和ALP。“一贯煎”或联合熊去氧胆酸治疗PBC小鼠疗效更好,为以后中西医联合治疗PBC提供了理论依据和技术支持。
To investigate the efficacy of ursodeoxycholic acid (UDCA) and “regular fried” in primary biliary cirrhosis in mice. The C57BL / 6 female mice injected with polyI: C were given UDCA, “consistent” and the two drugs combined treatment, analysis of different drug treatment group serum ALP, -GT changes, real-time fluorescent quantitative RT-PCR method Spleen mononuclear cells BCMA mRNA expression, and analysis of hepatic portal mononuclear cell infiltration rate. The results showed that the infiltration rate of mononuclear cells in hepatic portal area and the expression of BCMA mRNA in spleen mononuclear cells in PBC model rats were significantly higher than those in normal mice (P <0.05). The levels of ALP and -γGT were significantly decreased in different treatment groups compared with model control group (P <0.05), “consistent” or combined treatment can reduce the incidence of mononuclear cells in the hepatic portal area, downregulation of spleen mononuclear cells BCMAmRNA and ALP. “Always fried” or combined with ursodeoxycholic acid treatment of PBC mice better effect for the future of Chinese and Western medicine combined treatment of PBC provides a theoretical basis and technical support.