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目的 通过对与人类生活密切相关的多种野生、驯化和圈养动物携带SARS样病毒状况的研究 ,比较与野生动物密切接触人群和健康人群SARS病毒感染率 ,研究探索SARS病毒的来源。 方法 于 2 0 0 3年 5月起在广东等7个省自治区调查并采集动物咽漱液、鼻拭子和肛拭子样本 ,进行细胞培养、IFA、荧光RT -PCR检测 ;分析不同动物携带SARS样病毒状况。 结果 2 0 0只动物样本检出 10只动物携带SARS样病毒 ,其中主要是果子狸 (9只、阳性率为7 96% )。 1740名人员血清的SARSIgG抗体总阳性率为 5 0 6% ,其中野生动物销售人员最高 (4 5 45 % ) 结论 研究结果支持SARS病毒来源于动物 (果子狸 )的假设 ,并推测SARS病毒能从动物传播给人类。
OBJECTIVE: To investigate the SARS-associated virus status of many wild, domesticated and captive animals that are closely related to human life, and to compare SARS-virus infection rates with wild animals in close contact with healthy people. Methods Samples of animal pharyngeal swabs, nasal swabs and anal swabs were collected from 7 provinces in Guangdong and other provinces in May 2003 to carry out cell culture, IFA and fluorescence RT-PCR. SARS-like virus condition. Results In 200 animal samples, 10 animals were detected as SARS-like viruses, mainly civet cats (9, with a positive rate of 7 96%). The total positive rate of SARSIgG antibodies in 1740 seropositives was 56%, of which the highest was wildlife sales (4545%). Conclusions The findings support the hypothesis that the SARS virus is derived from animals (civet cats) and speculate that the SARS virus can Animals spread to humans.