运用表达谱芯片技术对慢性粒细胞白血病相关的癌基因、抑癌基因表达谱进行研究 对慢性粒细胞白血病及正常对照细胞进行总RNA抽提 ,纯化后的mRNA进行逆转录制备杂交探针 ,应用Biostar 10 2 4条人类全长基因cDNA芯片筛选与CML相关的基因 杂交信号用ScanArray 5 0 0 0扫描仪扫描 ,用ImaGene 3.0软件分析 ,发现有9个癌基因、4个抑癌基因同时在慢性粒细胞白血病异常表达 分析这些异常基因表达谱 ,对进一步全面了解发病机制提供科学依据 The use of expression profiling chip technology of chronic myeloid leukemia-related oncogene, tumor suppressor gene expression profile of chronic myeloid leukemia and normal control cells total RNA extraction, purified mRNA reverse transcription prepared hybridization probe, the application Biostar 10 24 human cDNA microarray cDNA screening and CML-related gene hybridization signal with ScanArray 500 Scanner scan, with ImaGene 3.0 software analysis found that 9 oncogenes, 4 tumor suppressor genes simultaneously in chronic Abnormal expression of myeloid leukemia Analysis of these abnormal gene expression profiles provides a scientific basis for further understanding of the pathogenesis