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采用不同ｐＨ 值沉淀法及超速离心获得提纯的水稻草矮病特异蛋白。提纯的Ｓ－ 蛋白具有典型的蛋白紫外吸收曲线，提纯产量为２８ ｍｇ／１００ ｇ 病叶。从ＳＤＳ－ ＰＡＧＥ 精提纯的Ｓ－ 蛋白免疫兔子上获得抗血清，经微量沉淀反应、琼脂双扩散测定，其效价分别为１∶１０２４ 和１∶２５６ 。获得的Ｓ－ 蛋白抗血清与提纯的Ｓ－ 蛋白及感病植株，在琼脂双扩散反应中，均有明显的沉淀线出现，对照健株及提纯的水稻草矮病毒则无反应。利用制备的Ｓ－ 蛋白抗血清经Ａ－ 蛋白酶联免疫吸附法检测，结果表明：Ｓ－蛋白抗血清与提纯Ｓ－ 蛋白及感病植株有专化性反应，且不同品种、不同部位、接种后不同发病时间的水稻病株中Ｓ－ 蛋白的积累量有差异，这就为ＲＧＳＶ 的检测提供了一种快速、简便的方法
Using different pH precipitation and ultracentrifugation to obtain purified rice grass dwarf disease-specific proteins. The purified S-protein has a typical protein UV absorbance curve with a purified yield of 28 mg / 100 g of diseased leaves. Antiserum was obtained from S-protein immunized rabbits purified by SDS-PAGE. The titer was 1:1024 and 1:256, respectively, by microprecipitation and agar double diffusion assay. The obtained S-protein antiserum, purified S-protein and susceptible plants showed obvious precipitation lines in the agar double diffusion reaction, but no reaction in the control plant and the purified rice grassy dwarf virus. The prepared S-protein antiserum was detected by A-protein enzyme-linked immunosorbent assay. The results showed that the S-protein antisera reacted specifically with the purified S-protein and susceptible plants, and different varieties, different parts, after inoculation The differences in S-protein accumulation in rice diseased plants at different stages of onset provided a quick and easy method for the detection of RGSV