农杆菌介导将美洲商陆抗病毒蛋白基因(pap)导入烟草云烟87品种的叶肉细胞,经过抗性筛选和鉴定,获得转基因植株.利用RT-半定量法分析了Y1、Y2和Y3转基因株系的pap表达,以及攻毒前后叶片多酚氧化酶(PPO)、超氧物歧化酶(SOD)活性和可溶性蛋白质含量.结果表明:pap在Y1和Y2植株中表达,Y3植株中未检测到pap表达;攻毒后,Y1和Y2叶片中可溶性蛋白质含量、PPO和SOD活性较高,而Y3叶片中可溶性蛋白质含量、PPO和SOD活性在三者中最低,与对照相似.讨论了转基因植株中pap表达与其生理变化和病毒抗性的关系. Agrobacterium-mediated transformation of pokeweed papaya into mesophyll cells of tobacco Yunyan 87 and screening of resistance and identification of transgenic plants.Analysis of Y1, Y2 and Y3 transgenic lines by RT-semi-quantitative analysis The expression of pap and the activities of PPO and SOD and the content of soluble protein in the leaves of Y1 and Y2 plants were detected.The results showed that pap was expressed in Y1 and Y2 plants but not in Y3 plants pap, respectively.The content of soluble protein, PPO and SOD in Y1 and Y2 leaves were higher after challenge, while soluble protein content, PPO and SOD activity in Y3 leaves were the lowest among the three, which were similar to the control Relationship between pap expression and physiological changes and virus resistance.