Stigma exsertion,a key determinant of the rice mating system,greatly contributes to the application of heterosis in rice.Although a few quantitative trait loci associated with stigma exsertion have been fine mapped or cloned,the underlying genetic architecture remains unclear.We performed a genome-wide association study on stigma exsertion and related floral traits using 6.5 million SNPs characterized in 533 diverse accessions of Oryza sativa.We identified 23 genomic loci that are significantly associated with stigma exsertion and related traits,three of which are co-localized with three major grain size genes GS3,GW5,and GW2.Further analyses indicated that these three genes affected the stigma exsertion by controlling the size and shape of the spikelet and stigma.Combinations of GS3 and GW5 largely defined the levels of stigma exsertion and related traits.Selections of these two genes resulted in specific distributions of floral traits among subpopulations of O.sativa.The low stigma exsertion combination gw5GS3 existed in half of the cultivated rice varieties;therefore,introducing the GW5gs3 combination into male sterile lines is of high potential for improving the seed production of hybrid rice.