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目的探讨复发性流产(RSA)病人及正常早孕妇女绒毛组织中FK506结合蛋白52(FKBP52)基因和蛋白的表达。方法选取2012年2月至2013年6月广西医科大学第一附属医院妇产科门诊就诊的30例RSA妊娠妇女(复发性流产组)及同期正常早孕妇女30例(对照组),收集其新鲜绒毛组织,分别应用实时定量聚合酶链反应(RT-PCR)及免疫组织化学方法检测两组绒毛组织中FKBP52基因和蛋白的表达。结果 FKBP52在两组妇女的绒毛组织中均有表达,但与对照组相比,复发性流产组凝胶成像系统半定量分析FKBP52 m RNA(1.484±0.198对1.109±0.292,P<0.05)及蛋白表达(91.8±5.3对68.2±8.4,P<0.01)均明显降低。结论 FKBP52的表达可能在胚胎着床和早期妊娠的维持中起重要作用,其表达降低可能与RSA的发病密切相关。
Objective To investigate the expression of FKB5052 (FKBP52) gene and protein in chorionic villi of recurrent spontaneous abortion (RSA) patients and normal early pregnant women. Methods Thirty cases of RSA pregnant women (recurrent spontaneous abortion) and 30 normal pregnant women (control group) were selected from the obstetrics and gynecology clinic of the First Affiliated Hospital of Guangxi Medical University from February 2012 to June 2013. The fresh Villi tissue, respectively, using real-time quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry to detect the expression of FKBP52 gene and protein in two groups of villi. Results FKBP52 was expressed in the villi of both groups. However, compared with the control group, FKBP52 m RNA (1.484 ± 0.198 vs 1.109 ± 0.292, P <0.05) and protein Expression (91.8 ± 5.3 vs 68.2 ± 8.4, P <0.01) were significantly lower. Conclusion The expression of FKBP52 may play an important role in the maintenance of embryo implantation and early pregnancy. The decreased expression of FKBP52 may be closely related to the pathogenesis of RSA.