目的评价染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣膜的细胞毒性。方法将L929细胞经亚甲基蓝介导光氧化处理去细胞猪主动脉瓣浸提液培养后,用MTT比色法测定其相对增殖率。将L929细胞与染料介导光氧化处理去细胞猪主动脉瓣直接接触培养,于不同时间观察细胞的生长状态。将L929细胞种植于染料介导的光氧化处理去细胞猪主动脉瓣表面,用扫描电镜观察其生长情况。结果染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣的性质稳定,细胞毒性程度为0～Ⅰ级。L929细胞与瓣膜材料直接接触培养生长良好,形态学无明显改变。结论染料亚甲基蓝介导的光氧化处理去细胞猪主动脉瓣的细胞相容性良好。 Objective To evaluate the cytotoxicity of dye methylene blue-mediated photooxidation of acellular porcine aortic valve. Methods L929 cells were treated with methylene blue - mediated photooxidation and then cultured in acellular aortic valve extract. The relative proliferation rate of L929 cells was measured by MTT assay. L929 cells were exposed to dye-mediated apo-oxidation of porcine aortic valves for direct contact culture, and the growth of cells was observed at different times. L929 cells were seeded on the surface of porcine aortic valve treated with dye-mediated photooxidation. The growth of L929 cells was observed by scanning electron microscopy. Results The dye-methylene blue-mediated photooxidation of porcine aortic valve acellular stability, cytotoxicity level 0 ~ Ⅰ. L929 cells and valve material in direct contact with culture grew well, no significant changes in morphology. Conclusion The dye-methylene blue-mediated photooxidation of acellular porcine aortic valve has good cytocompatibility.