本文作者对下列各种抗原激发保护性免疫的可能性进行了实验。 1.巴西日本圆线虫的分泌抗原(代谢抗原):从感染8天后的鼠肠内取出成虫,用磷酸盐缓冲盐水洗涤3次,在室温下保持在磷酸盐缓冲盐水中(更换2次)18小时,将虫体释出的可溶性产物经蒸馏水透析并低压干冻。2.虫体匀浆抗原:将成虫放在冰冷的匀 The authors tested the possibility that the following types of antigens would trigger protective immunity. 1. Secretion antigen (Metabolic antigen) of C. japonicus: Adult was taken from the rat intestine 8 days after infection, washed three times with phosphate buffered saline, and kept in phosphate buffered saline at room temperature (two changes) 18 Hour, the soluble products released by parasites were dialyzed against distilled water and lyophilized. 2 parasites antigen: the adults on the ice even