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为研究维生素D3受体(VDR)在正常人胎脑的分布,进而探讨1,25-二羟维生素D3[1,25-(OH)2D3]与正常人胎脑发育的关系,本实验按合法程序收集2～7月龄正常人胎脑,用免疫组织化学方法研究VDR在正常人胎脑的时空表达情况。结果显示:阳性产物在海马结构存在于胞核,3月龄出现表达的高峰,此后逐渐下降;在额叶皮质以及中脑部位的胞核和胞浆都可存在,其表达随胎龄增长。以上结果表明VDR在2～7月龄的人胎脑额叶皮质、海马结构和中脑有表达,且各部位的表达模式不同,提示1,25-(OH)2D3通过VDR可在人胎脑不同部位发育中发挥作用。
To investigate the distribution of vitamin D3 receptor (VDR) in normal human fetal brain and to explore the relationship between 1,25-dihydroxyvitamin D3 [1,25- (OH) 2D3] and normal fetal brain development, The program collected 2 to 7-month-old normal fetal brain, using immunohistochemistry to study the spatial and temporal expression of VDR in normal human fetal brain. The results showed that the positive products in the hippocampal structure exists in the nucleus, peaked at 3 months of age, and then gradually decreased; in the frontal cortex and midbrain nuclei and cytoplasm can exist, the expression increased with gestational age . The above results showed that VDR was expressed in the cortex, hippocampal formation and midbrain of the brain in 2 ~ 7 months of age, and the expression patterns of different parts were different, suggesting that 1,25- (OH) 2D3 could be expressed in human fetal brain Different parts of the development play a role.