目的:建立大鼠血浆中氯吡格雷羧酸代谢物SR26334检测的高效液相色谱方法,研究SR26334的药代动力学。方法:血浆经高氯酸沉淀,以ZORBAX SB-C18为色谱柱;流动相为乙腈-水-0.1%TFA(三氟乙酸),流速为1.0 ml/min;检测波长为230 nm(0 min～4.5 min)、280 nm(4.5 min～6 min)。结果:SR26334浓度在0.5 mg/L～50 mg/L范围内线性关系良好(r=0.9999);定量下限为0.5 mg/L;低、中、高三个浓度的相对回收率100.34%～101.78%;日内RSD(相对标准偏差)和日间RSD均小于10%。结论:本方法准确可靠、简便快速,适用于大鼠血浆SR26334浓度的测定。 OBJECTIVE: To establish a HPLC method for the determination of clopidogrel metabolite SR26334 in rat plasma to study the pharmacokinetics of SR26334. Methods: The plasma was precipitated by perchloric acid and the mobile phase consisted of acetonitrile-water-0.1% TFA (trifluoroacetic acid) at a flow rate of 1.0 ml / min on a ZORBAX SB-C18 column. The detection wavelength was 230 nm 4.5 min), 280 nm (4.5 min ~ 6 min). Results: The linear range of SR26334 was 0.5 mg / L ~ 50 mg / L (r = 0.9999). The lower limit of quantitation was 0.5 mg / L. The relative recoveries of SR26334 were 100.34% ~ 101.78% Intraday RSD (relative standard deviation) and daytime RSD were less than 10%. Conclusion: This method is accurate and reliable, simple and rapid, suitable for the determination of plasma concentration of SR26334 in rats.