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报道了用ＳＥＰ－ＰＡＫＣ１８小预柱固相萃取技术和反相高效液相色谱法测定复方甘草片中微量吗啡含量的方法。对固相萃取条件进行了研究，最终选择１０％甲醇溶液为清洗溶液，７０％甲醇溶液为洗脱溶液，基本消除了干扰组分对测定的影响，色谱图背景吸收小，基线平稳。色谱柱为μ－ＢｏｎｄａｐａｋＣ１８柱，流动相为０．１ｍｏｌ／ＬＮａＨ２ＰＯ４溶液－甲醇（５１），检测波长为２８６ｎｍ。平均回收率为（１０１．２±１．５）％，ＲＳＤ为１．５％。
A method for determination of trace morphine in Compound Liquorice Tablets by SEP-PAKC18 small column solid-phase extraction and reversed-phase high performance liquid chromatography was reported. The conditions of solid-phase extraction were studied. The final selection of 10% methanol solution as the cleaning solution, 70% methanol solution as the elution solution, the elimination of interference components on the determination of the impact of the chromatogram background absorption is small, the baseline was stable. The column was μ-Bondapak C18 column, the mobile phase was 0.1 mol / L NaH2PO4 solution-methanol (51) and the detection wavelength was 286 nm. The average recovery was (101.2 ± 1.5)% and RSD was 1.5%.