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目的观察噬菌体对阴沟肠杆菌所致皮下脓肿的抗感染作用,为噬菌体疗法应用于细菌性感染提供依据。方法家兔的侧背部皮下注射阴沟肠杆菌(4×107CFU/mL)造成皮下脓肿,且脓肿的持续时间应在4 d以上。在脓肿的部位注射噬菌体(1×109PFU/mL)后,测量脓肿面积的大小、计数脓肿内细菌和噬菌体的数量。结果噬菌体治疗组动物的脓肿面积和脓肿部位的细菌数量明显低于对照组(P<0.05),而噬菌体的数量虽高于皮下单独注射噬菌体的数量,但差异无统计学意义(P>0.05)。结论噬菌体对阴沟肠杆菌所致皮下脓肿的治疗是有效的,提示噬菌体疗法对阴沟肠杆菌的感染具有潜在的应用价值。
Objective To observe the anti-infective effect of bacteriophages on subcutaneous abscess caused by Enterobacter cloacae, and to provide basis for bacteriophage therapy for bacterial infection. Methods Subcutaneous injection of Enterobacter cloacae (4 × 107CFU / mL) on the dorsum of rabbits resulted in subcutaneous abscess, and the duration of abscess should be more than 4 days. After injection of phage (1 x 109 PFU / mL) into the abscess area, the size of the abscess area was measured and the number of bacteria and phage in the abscess was counted. Results The number of abscesses and abscess in the phage treatment group was significantly lower than that in the control group (P <0.05), while the number of phage was higher than that of the phage injected subcutaneously in the phage treatment group, but the difference was not statistically significant (P> 0.05) . Conclusion Bacteriophages are effective for the treatment of subcutaneous abscess caused by Enterobacter cloacae, suggesting that bacteriophage therapy has potential application value for Enterobacter cloacae infection.