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目的观察雌二醇(estradiol,E2)对小鼠外周血内皮祖细胞(endothelial progenitor cell,EPC)动员及损伤颈动脉内皮修复的效果。方法 C57/BL6小鼠15只,分为卵巢切除组(n=6),假手术组(n=4)及卵巢切除联合雌激素注射组(n=5)。卵巢切除组去势后给予雌激素治疗,随后建立小鼠颈动脉损伤模型,分别于1 d及3 d后流式细胞分析小鼠外周血EPC数量变化,伊文氏蓝染色评价7 d后颈动脉再内皮化面积。结果颈动脉损伤前各组外周血EPC数量:卵巢切除+雌激素组为(0.06±0.02)%、卵巢切除组为(0.03±0.01)%,未切除卵巢组为(0.06±0.02)%。颈动脉损伤后1、3 d,各组外周血EPC数量:卵巢切除+雌激素组为(0.42±0.14)%、(1.47±0.39)%,卵巢切除组为(0.13±0.02)%、(0.25±0.02)%,未切除卵巢组为(0.38±0.08)%、(0.71±0.17)%。颈动脉损伤7 d后卵巢切除组再内皮化面积较未切除卵巢组及卵巢切除+雌激素组减少[(28.3±13.5)%vs(69.5±14.1)%、(83.1±7.9)%;P<0.01]。结论雌二醇可动员小鼠外周血EPC,修复损伤血管内皮。
Objective To observe the effect of estradiol (E2) on the mobilization of endothelial progenitor cells (EPCs) and the injury of carotid artery endothelial cells in mice. Methods Fifteen C57 / BL6 mice were divided into ovariectomized group (n = 6), sham operation group (n = 4) and ovariectomized combined estrogen injection group (n = 5). Ovariectomized group after estrogen treatment, and then established a mouse carotid artery injury model, respectively, 1 d and 3 d after flow cytometry analysis of mouse peripheral blood EPC number, Evans blue staining evaluation of the carotid artery after 7 d Endothelialization area. Results The number of peripheral blood EPC in each group before carotid artery injury was (0.06 ± 0.02)% in ovariectomy plus estrogen group and (0.03 ± 0.01)% in ovariectomized group and (0.06 ± 0.02)% in non-excised ovary group. The numbers of EPCs in the peripheral blood of each group were (0.42 ± 0.14)% and (1.47 ± 0.39)% in ovariectomy + estrogen group and (0.13 ± 0.02)% and ± 0.02)%, and (0.38 ± 0.08)%, (0.71 ± 0.17)% in the group without ovariectomy. After 7 days of carotid artery injury, the area of re-endothelialization in ovariectomized group was significantly lower than that in non-excised ovariectomized group and ovariectomized + estrogen group (28.3 ± 13.5% vs 69.5 ± 14.1%, 83.1 ± 7.9%, P < 0.01]. Conclusion Estradiol mobilizes peripheral blood EPC in mice and repairs damaged vascular endothelium.