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目的 用高效液相色谱法研究刺五加制剂中 2个主要活性成分刺五加苷B、苷E的含量测定方法。方法KromasilODS柱 ,水 乙腈梯度流动相 ,流速 0 . 8mL·min-1,测定波长刺五加苷B 2 0 6nm ,刺五加苷E 2 2 0nm ,水杨酸作为内标 ,选择了固相萃取条件。结果 刺五加片中刺五加苷B、苷E的回收率范围分别是 90 . 4 %～ 96 . 8%和 87. 7%～ 93 .3% ;刺五加注射液中刺五加苷B、苷E的回收率范围分别是 96 .4 %～ 99. 8%和 95 . 7%～ 98. 5 %。线性范围分别是 4 .4 5～ 2 2 . 2 5 μg·mL-1(r=0 . 9998)和 5. 11～ 2 5 . 5 5 μg·mL-1(r=0 . 9997)。结论 该方法节约了清洗色谱系统的时间 ,提高了测定的灵敏度 ,提供了评价刺五加制剂质量的方法。
Objective To determine the content of the two main active components of Acanthopanax senticosus B and glycoside E by HPLC. METHODS Kromasil ODS column, acetonitrile gradient mobile phase, flow rate 0.8 mL·min-1, determination wavelength of eleutheroside B 2 0 6 nm, eleutheroside E 2 2 0 nm, salicylic acid as internal standard, solid phase selected Extraction conditions. Results The recoveries of Acanthopanax glycoside B and glycoside E in Acanthopanax senticosus tablets ranged from 90.4 to 96.8% and 87.7% to 93.3%, respectively; The recoveries of B and glycoside E ranged from 96.4% to 99.8% and 95.7% to 98.5%, respectively. The linear ranges were 4. 4 5 - 2 2 . 2 5 μg·mL -1 (r = 0.998) and 5. 11 - 25.5 5 μg·mL -1 (r = 0.997), respectively. Conclusion This method saves the time needed to clean the chromatographic system, improves the sensitivity of the assay, and provides a method for evaluating the quality of Acanthopanax senticosus preparations.