Transient expression of hepatitis B virus surface antigen (HBsAg) and human erythropoietin (hEPO) ge

来源 :Science in China(Series C:Life Sciences) | 被引量 : 0次 | 上传用户:lengyubo88
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The ability to deliver nonviral DNA directly into animals would provide major practical and economic benefits. To pursue this potential, as a preliminary approach, the expression in mammary epithelial cells with nonviral DNA encoding HBsAg and hEPO was investigated by in vivo delivery directly into mammary gland. Either the bovine αs1 casein HBsAg or the WAP hEPO gene construct was introduced into mammary glands of dose by mammary artery injection. A single injection of 10 11 copies of DNA construct was implemented for a doe. Transient expression of the constructs in milk collected from milk letdown by induction up to 19 d after injection was measured with an hEPO ELISA kit from Boehringer Mannheim (Cat. No.1 693 417) for rhEPO, and a HBsAg ELISA kit (Abbott Lab, USA) for rHBsAg. The treated animals showed mean concentration of recombinant molecules at 16 μg/L for rHBsAg, and 2 mg/L for rhEPO in milk. The pursuit of potential, as a preliminary approach, the expression in mammary epithelial cells with nonviral DNA encoding HBsAg and hEPO was investigated by in vivo delivery directly into mammary gland . Either the bovine αs1 casein HBsAg or the WAP hEPO gene construct was introduced into mammary glands of dose by mammary artery injection. A single injection of 10 11 copies of DNA construct was implemented for a doe. Transient expression of the constructs in milk collected from milk letdown by induction up to 19 d after injection was measured with an hEPO ELISA kit from Boehringer Mannheim (Cat. No. 1 693 417) for rhEPO, and a HBsAg ELISA kit (Abbott Lab, USA) for rHBsAg. mean concentration of recombinant molecules at 16 μg / L for rHBsAg, and 2 mg / L for rhEPO in milk.
其他文献
EnzymeDynamicStudyonCalmodulinCa++┐Mg++┐ATPaseSystemofRedBloodCelsInhibitedbyα┐anordrinandProbimaneLuDayongChenEnhong*CaoJing... EnzymeDynamicStudyonCalmodulinCa ++ ┐Mg ++ ┐ATPaseSystemofRedBloodCelsInhibitedbyα┐anordrinandProbimaneLuDayongChenEnhong * C
许多抗癌均有严重副作用,尚能增强抗癌剂的抗癌活性。津村药理研究所油田正树在并用丝裂霉素 C(MMC)和津村顺天堂中药制剂的动物实验后,发现在116种中药方剂中,以十全大补汤
作者前已报道人参总皂甙能缩小家兔实验性心肌梗塞范围,并探讨其作用原理可能与减慢心率、降低椎动脉和股动脉血管阻力、节约全身耗氧和增加耐缺氧力等作用有关。本文报道它
“速效小儿消疾丸”于一九八五年二月在山西临汾市通过鉴定。“速效小儿消疾丸”是山西省临汾中药厂根据77岁名老中医孟宪瑞先生所献的祖传秘方,利用传统的炮制工艺,结合现
本实验结果初步表明:黄芪、白山云芝注射液对烧伤后大白兔的中性白细胞吞噬及杀菌功能的回升有作用,伤后第一周给药组动物的白细胞吞噬、杀菌功能开始回升,第二周达到高峰,第
吡硫霉净(Sodium 2-mercaptopyridine-N-Oxide)是一种抗霉菌药,其含量测定方法很多,主要有氯化亚钛法、极谱法和比色法。上述方法中以比色法较为简便且具有专一性。最近有用
建国以来,由于党中央对中医中药的重视,中药事业取得了很大的发展。在国家对私营工商业的改造时,中药店合并为规模较大的国营药店,中医中药人员都吸收到国营企事业单位或联
方剂:党参50克,当归50克,川芎30克,黄芪30克,芥穗25克,陈皮30克,防风25克、升麻20、白术25克、艾叶45克,红花20克、甘草20克。水煎候温加入白酒60毫升,灌服。 Formula: Cod
了解福建棘隙吸虫体被的超微形态学特征。采用扫描电镜观察实验豚鼠体内福建棘隙吸虫的体表结构:口吸盘有3种棘,其中位于口吸盘基部背、腹侧分布4例棘。腹吸盘有18枚棘绕围孔部分布
备受中国企业界关注的中国最有价值品牌研究1999年度报告近日揭晓,红塔山以433亿元的品牌价值继续名列榜首。这项研究是忡国质量万里行》杂志社和北京名牌资产评估事务所借鉴