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目的比较日本血吸虫非适宜宿主东方田鼠和SD大鼠特异性DNA序列的差异。方法提取东方田鼠、SD大鼠、C57BL/6小鼠和KM鼠肝脏DNA,根据东方田鼠特异性DNA序列片段(GenBank登录号:AF277394)设计引物,PCR方法扩增以上4种鼠的基因组特异性DNA,琼脂糖凝胶电泳鉴定特异条带,纯化后的PCR产物克隆到pGEM-T Easy载体,进行DNA序列分析。结果 PCR扩增东方田鼠、SD大鼠DNA分别得到520bp左右的特异扩增片段,同源性为99%。C57BL/6小鼠和KM鼠没有扩增出此特异片段。结论日本血吸虫非适宜宿主东方田鼠和SD大鼠之间特异性DNA序列有高度的同源性。
Objective To compare the differences of the specific DNA sequences of Microtus fortis and SD rats not suitable for Schistosoma japonicum. Methods The liver DNA of Microtus fortis, SD rats, C57BL / 6 mice and KM mouse were extracted and the primers were designed according to the DNA sequence of Microtus fortis (GenBank accession number: AF277394). The genomic specificity DNA, agarose gel electrophoresis to identify specific bands, the purified PCR product was cloned into pGEM-T Easy vector for DNA sequence analysis. Results The specific amplified fragment of about 520bp was obtained by PCR amplification of DNA from Microtus Fortis and SD rats, respectively. The homology was 99%. C57BL / 6 mice and KM mice did not amplify this specific fragment. Conclusion The specific DNA sequences between T. voleus and SD rats are not homologous to the non-suitable host Schistosoma japonicum.