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目的研究基质金属蛋白酶(MMPs)在椎间盘退变中的作用及愈骨胶囊对兔椎间盘退变模型MMPs的影响。方法将成年新西兰白兔按随机数字法分为正常组、模型组、实验组,每组20只。用手术方法建立兔椎间盘退变模型。实验组给予0.15 g·kg~(-1)愈骨胶囊,正常组和模型组给予等量纯化水,连续给药4周。用免疫组化法和免疫印迹法检测兔腰椎间盘组织中MMP-3的表达,依据试剂盒说明检测血清中钙(Ca)和碱性磷酸酶(ALP)含量,双能X线吸收法(DXA)测定椎间盘组织中骨密度(BMD)和骨矿(BMC)含量。结果模型组,MMP-3表达显著高于对照组(P<0.05),而愈骨胶囊组,MMP-3表达显著低于模型组(P<0.05)。模型组,血清Ca和ALP、BMD和BMC含量显著低于对照组;而愈骨胶囊组,血清Ca和ALP、BMD和BCM含量显著高于模型组(P<0.05)。结论愈骨胶囊可降低MMP-3表达,促进钙盐形成,加速钙离子沉积,有效改善椎间盘退变。
Objective To investigate the role of matrix metalloproteinases (MMPs) in the degeneration of intervertebral disc and the effect of jiangu capsule on MMPs in degenerative disc model of rabbit. Methods Adult New Zealand White rabbits were randomly divided into normal group, model group and experimental group according to random number method. Rabbit disc degeneration model was established by operation method. The experimental group was given 0.15g · kg -1 Jinu capsule, and the normal group and the model group were given the same amount of purified water for continuous administration for 4 weeks. The expression of MMP-3 in rabbit lumbar intervertebral disc tissue was detected by immunohistochemistry and Western blotting. The levels of Ca (superscript +) and alkaline phosphatase (ALP) in serum were detected by kit instructions. The dual-energy X-ray absorptiometry ) To determine the bone mineral density (BMD) and bone mineral (BMC) content in the intervertebral disc tissue. Results The expression of MMP-3 in the model group was significantly higher than that in the control group (P <0.05), while the expression of MMP-3 in the Yujian capsule group was significantly lower than that in the model group (P <0.05). The content of serum Ca, ALP, BMD and BMC in the model group was significantly lower than that in the control group. The content of Ca, ALP, BMD and BCM in the group of Yugu capsule was significantly higher than that of the model group (P <0.05). Conclusion Yujian capsule can reduce the expression of MMP-3, promote the formation of calcium salt, accelerate the deposition of calcium ions and effectively improve the degeneration of intervertebral discs.