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全球流行病学研究显示,饮用咖啡与T2DM患病率负相关。而人胰岛淀粉样多肽(hIAPP)错折叠被视为T2DM的诱因之一。咖啡提取物含有3种活性成分:咖啡因,咖啡酸(CA)和绿原酸(CGA)。该研究通过硫磺素T荧光染色,透射电子显微镜、圆二色谱、光诱交联、动态光散射和MTT细胞生存分析,考察上述3种主要成分以及二氢咖啡酸(DHCA,CGA和CA的
Global epidemiological studies have shown that drinking coffee is negatively correlated with the prevalence of T2DM. Human amylin amyloid peptide (hIAPP) misfolding is considered one of the causes of T2DM. The coffee extract contains three active ingredients: caffeine, caffeic acid (CA) and chlorogenic acid (CGA). In this study, the above three main components as well as dihydrofolate (DHCA, CGA and CA) were investigated by thioflavin T staining, transmission electron microscopy, circular dichroism, photoinduction, dynamic light scattering and MTT cell survival analysis