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目的 测定 5个曼氏血吸虫吡喹酮抗性株和 5个曼氏血吸虫吡喹酮敏感株对吡喹酮的敏感性 ,了解用不同方法配制吡喹酮对化疗效果的影响。方法 用不同虫株尾蚴分别感染 CD1 小鼠 ,在感染后第 5 8— 6 0天以 3× 2 0 0 m g/ kg微粒化吡喹酮作灌胃治疗 ,化疗后第 2 1天剖杀小鼠收集成虫 ,比较减虫率。用同一虫株的尾蚴感染 CD1 小鼠 ,感染后第 5 8— 6 2天以 5× 10 0 mg/ kg微粒化、未微粒化及加甘油未微粒化吡喹酮治疗感染小鼠 ,化疗后第 2 1天剖杀小鼠收集成虫 ,比较减虫率。结果 5个吡喹酮敏感株的减虫率为 91.8%一 10 0 % ;5个吡喹酮抗性株的减虫率为 5 9.6 %一 74 .4 %。 5× 10 0 m g/ kg微粒化吡喹酮组减虫率为 73.0 % ,而未微粒化组和加甘油组为 4 7.9%和 5 0 .0 %。结论 5个曼氏血吸虫抗性株对吡喹酮的敏感性显著低于 5个敏感株 ;吡喹酮配制方法影响化疗结果 ,微粒化吡喹酮化疗效果优于另 2种未微粒化吡喹酮。
Objective To determine the sensitivity of praziquantel and five praziquantel - sensitive strains of schistosoma mansoni to praziquantel in five schistosoma mansoni strains and to study the effects of praziquantel on the efficacy of chemotherapy using different methods. Methods CD1 mice were infected with different cercaria strains of cercariae respectively, and intragastrically treated with 3 × 200 mg / kg of praziquantel intragastrically on the days 5-8-10 after infection. The mice were sacrificed on the 21st day after chemotherapy Rats collected adults, more worm reduction rate. CD1 mice were infected with the cercariae of the same strain and infected with 5 × 10 0 mg / kg particles on day 5-8 and infected with praziquantel without glycerol glycerol. After chemotherapy, On the 21st day, mice were sacrificed and adults were collected to compare their worm reduction rates. Results The worm reduction rates of the five praziquantel-sensitive strains were 91.8% -10.0%. Five praziquantel-resistant strains had a worm reduction rate of 59.6% -74.4%. The worm reduction rate of 5 × 10 0 m g / kg micronized praziquantel was 73.0%, while it was 49.9% and 50.0% for the non-micronized and glycerinated groups. Conclusions The sensitivity of five strains of Schistosoma mansoni to praziquantel was significantly lower than that of five sensitive strains. The preparation method of praziquantel affected the results of chemotherapy. The efficacy of micronized praziquantel was better than that of two other non-micronized praziquantel ketone.