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用大麦品种86-166的未成熟胚为外植体,通过离体培养获得81个体细胞无性系,研究了R_2代抽穗期、株高、单株穗数、旗叶长、旗叶宽、穗颈长、穗长和结实率等8个农艺性状的变异规律。结果表明,大麦体细胞无性系二代存在着广泛的性状变异,一般株系间变异大于株系内变异,而在株系内个体间的表型差异性上,可出现高度纯合、一致和不一致3种情况。按株系统计,8个农艺性状的平均变异频率为65.39％,其中稳定变异为48.65％,分离变异为16.75％。以抽穗期、旗叶长、旗叶宽、穗颈长和穗长的变异频率较高,达80％以上,其次为株高和单株穗数,各为40.74％和29.63％;结实率的变异频率为最低,仅14.81％。各农艺性状均具有明显的变异趋向,般表现为抽穗期延迟,植株变矮,穗数增加,穗变长,穗颈变短,叶片变大,结实率降低。
Using immature embryos of barley variety 86-166 as explants, 81 somatic clones were obtained through in vitro culture. The heading date, plant height, number of panicle per plant, length of flag leaf, width of flag leaf, Neck length, spike length and seed setting rate of 8 agronomic traits. The results showed that the barley second generation of somatic clones had a wide range of trait variation, the variation between the general lines was greater than the intra-line variation, and the phenotypic differences among the individuals in the lines could be highly homozygous and consistent Inconsistent three kinds of situations. The average frequency of variation of eight agronomic traits was 65.39% by strain system, among which the stable variation was 48.65% and the segregation variation was 16.75%. The variation frequency of heading stage, flag leaf length, flag leaf width, panicle neck length and spike length was higher than 80%, followed by plant height and panicle per plant, which were 40.74% and 29.63% respectively. The seed setting rate The frequency of variation was the lowest, only 14.81%. Agronomic traits all have obvious tendency of variation. The general appearance is the delay of heading stage, the shorter plants, the more spikes, the longer spikes, the shorter panicles, the bigger leaves and the lower seed setting rate.