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目的研究调节性T细胞在膀胱癌患者组织和血液中的表达及其临床意义。方法收集膀胱癌患者组织标本及其血液标本各20例,应用流式细胞术检测T淋巴细胞亚群的分布,实时定量PCR技术检测膀胱癌组织及癌旁组织细胞因子转化生长因子-β(TGF-β),白介素(IL)-4、-10、-12、-17和干扰素-γ(IFN-γ)等表达水平,并分析调节性T细胞的表达与膀胱癌临床病理特征的关系。结果与膀胱癌癌旁组织标本相比(3.58±0.31)%,在膀胱癌中调节性T细胞显著增多(29.10±0.75)%(P<0.01),浸润性膀胱癌组织中CD4+CD25+Treg细胞的数量与肿瘤分级和分期具有显著相关性(r=0.73,P<0.05),而且抑制性细胞因子TGF-β和IL-10的表达量显著增多(P<0.01),IL-17表达亦增多(P<0.01)。与健康对照组(5.2±0.63)%相比,膀胱癌患者外周血中调节性T细胞亦显著增多(15.68±0.54)%,且膀胱癌组织中的调节性T细胞阳性细胞百分比较外周血中更多(P<0.01)。结论膀胱癌患者组织及外周血均表现免疫抑制状态,膀胱癌组织的调节性T细胞阳性表达数量与临床病理特征具有相关性。
Objective To study the expression of regulatory T cells in the tissue and blood of patients with bladder cancer and its clinical significance. Methods Twenty cases of bladder cancer tissue specimens and their blood samples were collected. The distribution of T lymphocyte subsets was detected by flow cytometry. The expression of TGF - β (TGF - β) in bladder cancer tissues and adjacent tissues was detected by real - time quantitative polymerase chain reaction β, IL-4, -10, -12, -17 and IFN-γ, and to analyze the relationship between the expression of regulatory T cells and clinicopathological features of bladder cancer. Results Compared with the adjacent tissues of bladder cancer (3.58 ± 0.31)%, the number of regulatory T cells was significantly increased in bladder cancer (29.10 ± 0.75)% (P <0.01), while the expression of CD4 + CD25 + Treg in invasive bladder cancer The number of cells was significantly correlated with tumor grade and stage (r = 0.73, P <0.05), and the expression of inhibitory cytokines TGF-β and IL-10 was significantly increased (P <0.01) Increased (P <0.01). Compared with the healthy control group (5.2 ± 0.63)%, the number of T regulatory cells in peripheral blood of bladder cancer patients was significantly increased (15.68 ± 0.54)%, and the percentage of T regulatory cells in bladder cancer tissues was significantly higher than that in peripheral blood More (P <0.01). CONCLUSION: The immunosuppressive status of bladder cancer tissues and peripheral blood are all shown. The number of Treg positive cells in bladder cancer is correlated with clinicopathological features.