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目的探讨5-羟色胺4(5-HT4)受体在先天性巨结肠(HD)和先天性巨结肠同源病(HAD)中的表达,并研究能否将此检测应用于HD和HAD的术前鉴别诊断。方法共收集HD标本25例(HD组)、HAD标本20例(HAD组)和正常结肠标本15例(正常对照组)。应用免疫组织化学SP法染色,分析5-HT4受体表达的部位及肠管不同部位5-HT4受体表达水平强度的差异,采用t检验比较其表达是否存在差异性。结果 HD组5-HT4受体阳性指数在远端黏膜层较近端表达下降,肌间神经丛无表达;HAD组远端5-HT4受体阳性指数在黏膜层和肌间神经丛中均显著低于近端。与正常对照组比较,HD组和HAD组5-HT4受体阳性指数在黏膜及肌间神经丛表达均下降。结论 5-HT4受体在HD及HAD中的表达水平可作为术前鉴别诊断的参考指标之一,也为临床治疗提供新的参考指标。
Objective To investigate the expression of serotonin 4 (5-HT4) receptor in Hirschsprung’s disease and Hirschsprung’s disease, and to investigate the feasibility of applying this assay to HD and HAD Pre-differential diagnosis. Methods Totally 25 HD specimens (HD group), 20 HAD specimens (HAD group) and 15 normal colon specimens (normal control group) were collected. The expression of 5-HT4 receptor and the expression of 5-HT4 receptor in different parts of intestine were analyzed by SP immunohistochemical staining. The differences of the expression of 5-HT4 receptor were analyzed by t test. Results The positive index of 5-HT4 receptor in HD group was lower than that in the proximal mucosa, but was not expressed in myenteric plexus. The positive index of distal 5-HT4 receptor in HAD group was significantly higher in mucosa and myenteric plexus Lower than proximal. Compared with the normal control group, the expression of 5-HT4 receptor positive index in both HD and HAD groups decreased in the mucosa and myenteric plexus. Conclusions The expression level of 5-HT4 receptor in HD and HAD can be used as a reference index for the differential diagnosis of preoperative diagnosis. It also provides a new reference index for clinical treatment.