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目的:建立子宫内膜异位症动物模型,探索PRL-3抑制剂对SD大鼠异位病灶的影响。方法:选取36只雌性SD大鼠,将自体子宫内膜移植至腹壁造模,免疫组化实验鉴定病灶内膜的上皮及间质组织。内异症大鼠腹腔注射PRL-3抑制剂,按给药浓度分组:对照组(0mg/kg)、低剂量组(0.1mg/kg)、中剂量组(1mg/kg)和高剂量组(10mg/kg),比较处理前后各组病灶情况。结果:SD大鼠自体子宫内膜移植建模存活率为86.1%,存活大鼠的造模成功率为92.5%。SD大鼠各组处理后对比处理前,对照组病灶明显增大(P<0.05),低剂量组和中剂量组病灶大小无明显变化,高剂量组病灶显著缩小(P<0.05)。高剂量组的病灶显著小于对照组(P<0.05)。结论:SD大鼠内异症模型可用于内异症的体内实验研究,是一种操作性强、重复性好且成本低的内异症模型。一定剂量的PRL-3抑制剂可抑制SD大鼠自体异位内膜病灶的生长;PRL-3抑制剂对内异症的靶向治疗有一定的潜在价值。
Objective: To establish an animal model of endometriosis and explore the effect of PRL-3 inhibitor on ectopic lesions of SD rats. Methods: Thirty-six female Sprague-Dawley rats were selected and their autologous endometrium was transplanted to the abdominal wall for modeling. The epithelial and interstitial tissues of the lesion intima were identified by immunohistochemistry. Rats with endometriosis were injected intraperitoneally with PRL-3 inhibitor. The rats in control group (0mg / kg), low dose group (0.1mg / kg), middle dose group (1mg / kg) and high dose group 10mg / kg), compared before and after treatment of lesions in each group. Results: The survival rate of autologous endometrial transplantation in SD rats was 86.1%, and that of surviving rats was 92.5%. Before treatment, the number of lesions in control group was significantly increased (P <0.05), but there was no significant change in the size of the lesion in the low dose group and the middle dose group (P <0.05). High-dose group of lesions was significantly smaller than the control group (P <0.05). Conclusion: SD rat model of endometriosis can be used for in vivo experimental study of endometriosis, which is a kind of endometriosis model with good operability, good repeatability and low cost. A certain dose of PRL-3 inhibitor can inhibit the growth of autologous ectopic endometrial lesions in SD rats. PRL-3 inhibitor has some potential value for the targeted therapy of endometriosis.