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目的研究自噬特异性标志物Beclin-1在肺腺癌发生、发展中的变化及其对术后患者预后的影响。方法采用反转录-聚合酶链反应(RT-PCR)及Western blot法分别检测108例正常肺组织、肺不典型腺瘤样增生(AAH)、肺原位腺癌、肺微浸润性腺癌及浸润性腺癌组织标本中自噬特异性标志物Beclin-1在基因及蛋白水平的表达,比较Beclin-1在不同组织中及不同病理分期肺腺癌患者肿瘤组织中的变化,分析其对肺腺癌患者术后预后的影响。结果肺腺癌Beclin-1基因及蛋白水平的表达均显著低于正常肺组织(P<0.05),浸润性肺腺癌组织表达最低;早期肺腺癌(Ⅰ~Ⅱ期)患者肿瘤组织Beclin-1的表达高于中晚期肺腺癌(Ⅲ~Ⅳ期)患者(P<0.05)。肺腺癌伴淋巴结转移者肿瘤组织Beclin-1的表达低于淋巴结阴性患者(P<0.05)。肺腺癌肿瘤组织Beclin-1阳性表达患者术后生存时间显著高于Beclin-1阴性表达者(P<0.05)。结论 Beclin-1的表达与肺腺癌的发生、发展相关;肺腺癌Beclin-1的表达缺失预示肺腺癌患者术后生存率较差。Beclin-1可能成为预测肺腺癌患者术后预后的重要标志物。
Objective To investigate the changes of autophagy-specific Beclin-1 in the occurrence and development of lung adenocarcinoma and its effect on the prognosis of patients after operation. Methods Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect 108 normal lung tissues, atypical adenomatous hyperplasia (AAH), adenocarcinoma of the lung, microinvasive adenocarcinoma of the lung and The expression of Beclin-1, a specific marker of autophagy in invasive adenocarcinoma, was compared with that of Beclin-1 in different tissues and in different stages of lung adenocarcinoma. The expression of Beclin- Postoperative prognosis of cancer patients. Results The expression of Beclin-1 gene and protein in lung adenocarcinoma was significantly lower than that in normal lung tissue (P <0.05), and the lowest in infiltrative lung adenocarcinoma. The expression of Beclin- 1 in patients with advanced lung adenocarcinoma (stage Ⅲ ~ Ⅳ) (P <0.05). The expression of Beclin-1 in lung adenocarcinoma with lymph node metastasis was lower than that in lymph node-negative patients (P <0.05). The survival time of Beclin-1 positive patients with lung adenocarcinoma was significantly higher than that of Beclin-1 negative patients (P <0.05). Conclusions The expression of Beclin-1 is correlated with the occurrence and development of lung adenocarcinoma. The loss of Beclin-1 expression in lung adenocarcinoma indicates that the survival rate of patients with lung adenocarcinoma is poor. Beclin-1 may be an important marker for predicting postoperative prognosis of patients with lung adenocarcinoma.