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为了解郑州铁路地区病毒性肝炎患者中乙型病毒性肝炎（乙肝）的感染状况及其流行病学特点，本文对郑州铁路中心医院１９９１年７月～１９９２年６月住院的４６０名病毒性肝炎患者，采用ＲＩＡ、ＥＬＩＳＡ、ＤｉｇＨＢｖ－ＤＮＡ探针及ＰＣＲ技术进行乙肝病原学诊断，结果表明：４６０例患者中２８９例为乙肝感染，占６２．８３％，其中１７６例为单纯感染，１１３例为混合感染。２８９例中男性１９１人，女性９８人，男女之比为１．９５：１。患者中半年内有输血、手术或注射、针刺史者共计１６１倒，占总数的５５．７１％。随机抽取６２份乙肝患者尿液，采用ＤｉｇＨＢｖ－ＤＮＡ探针，检出阳性１０例，捡出率１６．１３％。
In order to understand the infectious status of hepatitis B virus (HBV) and its epidemiological characteristics in patients with viral hepatitis in Zhengzhou Railway, 460 viral hepatitis patients admitted to Zhengzhou Railway Central Hospital from July 1991 to June 1992 Patients, using RIA, ELISA, DigHBv-DNA probe and PCR technology for the diagnosis of hepatitis B, the results showed that: 460 cases of 289 cases of hepatitis B infection, accounting for 62.83%, of which 176 cases were simple infection, 113 cases were Mixed infection. There were 191 males and 98 females in 289 cases, the ratio of male to female was 1.95: 1. Patients within six months of blood transfusion, surgery or injection, a total of 161 puncture history, accounting for 55.71% of the total. Sixty-two urine samples of patients with hepatitis B were randomly selected and 10 cases were positive with DigHBv-DNA probe. The detection rate was 16.13%.