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目的 :研究肿瘤坏死因子 - α(TNF- α)在中耳胆脂瘤组织中的表达 ,探讨其在中耳胆脂瘤骨质破坏中的作用。方法 :采用免疫组织化学 SABC染色法和计算机图像定量分析法 ,对 2 5例中耳胆脂瘤和 10例正常外耳道皮肤中 TNF- α的表达情况进行观察。结果 :TNF- α不仅在胆脂瘤上皮的各层细胞胞浆中 ,而且在皮下组织的炎性细胞中都有高度表达。定量分析显示胆脂瘤上皮中 TNF- α的含量显著高于外耳道皮肤 (P <0 .0 5 )。结论 :TNF-α可能是胆脂瘤引起骨质破坏的一个因素。
Objective: To study the expression of tumor necrosis factor - α (TNF - α) in cholesteatoma of middle ear and its role in bone destruction of middle ear cholesteatoma. Methods: Immunohistochemical SABC staining and computer image quantitative analysis of 25 cases of middle ear cholesteatoma and 10 cases of normal external auditory canal in the skin of TNF-α expression was observed. Results: TNF-α was highly expressed not only in cytoplasm of cholesteatoma but also in inflammatory cells of subcutaneous tissue. Quantitative analysis showed that the content of TNF-α in cholesteatoma epithelium was significantly higher than that of the external auditory canal (P <0.05). Conclusion: TNF-α may be a factor of bone destruction caused by cholesteatoma.