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本文根据生物碱与酸性染料形成有色络合物的原理,选用了溴甲酚绿为络合剂,用比色法测定生物碱含量,并与《药典》85版规定的测定方法相比较,结果此法稳定,重现性好。
Based on the principle of the formation of colored complexes between alkaloids and acid dyes, bromocresol green was selected as the complexing agent and the alkaloid content was determined by colorimetric method and compared with the determination method specified in the Pharmacopoeia 85 Edition. This method is stable and reproducible.