苯巴比妥是常见的安眠镇静药,在我国用其投毒和自杀的案件时有发生.过去鉴定苯巴比妥中毒的案件,多以胃、肝、血、肺、肾、尿为主要检材,而以眼玻璃体或骨头作为分析材料的未见报导.作者给狗口服不同量的苯巴比妥药物(治疗量、中毒量和致死量),并解剖提取眼玻璃体、骨头、肌肉以及其它内脏组织,用直接提取法提取,柱层析净化,再用TLC、GC、 Phenobarbital is a common sleep-depressant, which has occurred in our country with its poisoning and suicide cases.In the past, the cases of phenobarbital poisoning were mostly stomach, liver, blood, lung, kidney and urine However, no reports of ocular vitreous or bony materials have been reported. The authors orally administered different amounts of phenobarbital to the dogs (dose, toxicity and lethal dose) and anatomy of the ocular vitreous, bone, muscle and Other visceral tissues were extracted by direct extraction and purified by column chromatography. TLC, GC,