将经虎杖水提取物处理的HIV-1接种于MT-4细胞,结果未见细胞变性,而且培养上清液中无逆转录酶(RT)活性和感染力。经荧光抗体法与电子显微镜观察,在培养细胞中未见HIV-1抗原与病毒粒子。以PCR法也未能检出前病毒DNA.由此认为,虎杖的水提取物作用于病毒增殖的早期。对MT-4细 When HIV-1 treated with Polygonum cuspidatum extract was inoculated into MT-4 cells, no cell degeneration was observed, and there was no reverse transcriptase (RT) activity and infectivity in the culture supernatant. As observed by fluorescent antibody and electron microscopy, HIV-1 antigen and virus particles were not seen in cultured cells. The proviral DNA could not be detected by the PCR method. It is considered that the aqueous extract of Polygonum cuspidatum acts on the early stage of virus proliferation. Fine to MT-4