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Xa21是已经分离克隆的一个具有广谱抗性的水稻白叶枯病抗性基因,根据已克隆的白叶枯病抗性基因Xa21外显子Ⅱ序列设计特异性引物对云南3种野生稻及其他稻种进行PCR扩增。结果表明,只有普通野生稻(景洪普通野生稻和元江普通野生稻)及长雄野生稻中扩增到了长400bp的目的片段,而疣粒野生稻和药用野生稻及栽培稻中均没有扩增到目的片段。通过序列比较发现所克隆的序列同长雄野生稻的氨基酸序列变化是随机的。
Xa21 is a broad-spectrum resistance rice bacterial blight resistance gene which has been isolated and cloned. According to the sequence of Xa21 exon Ⅱ of cloned bacterial leaf blight resistance gene, Other rice varieties for PCR amplification. The results showed that only 400 bp of the target fragment was amplified from common wild rice (Jinghong and Yuanjiang common wild rice) and Changhong wild rice, while neither warty wild rice nor medicinal wild and cultivated rice Amplified to the desired fragment. The amino acid sequences of the cloned sequences and that of the longan (Oryza sativa) hybrids were found to be random.