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目的:观察牛膝总皂苷(TSA)对兔膝骨关节炎(OA)模型滑膜组织的影响。方法:将40只实验兔分为正常组8只、OA模型组32只,OA造模成功后将OA模型组分为OA模型阳性对照组、OA模型空白组、OA模型TSA低剂量组、OA模型TSA中剂量组、OA模型TSA高剂量组,各6只,分别予硫酸氨基葡萄糖、蒸馏水、TSA低剂量、TSA中剂量、TSA高剂量灌胃2个月后进行关节粘连改善度测定、滑膜组织病理HE染色、滑膜炎KRENN评分、β-catenin蛋白免疫组化检测,及关节液TNF-1,IL-1β,MMP-3含量检测。结果:实验兔OA模型造模成功后灌胃2个月,与OA模型空白组关节粘改善度比较,OA模型阳性对照组和OA模型TSA低、中、高剂量组差异有统计学意义(P<0.05),OA模型阳性对照组与OA模型TSA高剂量组比较差异有统计学意义(P<0.01);滑膜HE染色和滑膜炎评分比较,OA模型空白组见大量滑膜组织炎细胞浸润、纤维组织增生以及滑膜细胞增生、排列紊乱,滑膜组织周边可见较多毛细血管增生,呈重度滑膜炎表现,OA模型阳性对照组见中度滑膜组织炎细胞浸润、纤维组织增生以及滑膜细胞增生、排列不规则,滑膜组织周边可见少量毛细血管增生,呈中度滑膜炎表现,OA模型TSA高剂量组见轻度滑膜组织炎细胞浸润、纤维组织增生以及滑膜细胞增生、排列尚规则,滑膜组织周边可见微量毛细血管增生,呈轻度滑膜炎表现;β-catenin蛋白免疫组化比较,OA模型阳性对照组和OA模型TSA高剂量组与OA模型空白组比较,差异有统计学意义(P<0.01),OA模型阳性对照组与OA模型TSA高剂量组比较差异有统计学意义(P<0.05);各组与正常组比较TNF-1,IL-1β,MMP-3指标差异有统计学意义(P<0.05),OA模型TSA高剂量组与OA模型阳性对照组比较差异无统计学意义(P>0.05),两者与OA模型空白组比较差异有统计学意义(P<0.01)。结论:牛膝总皂苷能有效抑制OA滑膜炎症,改善关节粘连度,降低滑膜组织β-catenin蛋白表达以及减少关节液TNF-1,IL-1β,MMP-3因子含量,其作用机理有待进一步探索。
Objective: To observe the effect of total knee joint saponin (TSA) on the synovial tissue of rabbit knee osteoarthritis (OA) model. Methods: 40 rabbits were divided into normal group (n = 8) and OA model group (n = 32). OA model was divided into two groups: OA model positive control group, OA model blank group, OA model TSA low dose group, Model TSA middle dose group and OA model TSA high dose group, 6 rats in each group were treated with glucosamine sulfate, distilled water, low dose TSA, middle dose TSA and high dose TSA for 2 months respectively. The histopathological HE staining, KRENN synovitis score, β-catenin protein immunohistochemistry and TNF-α, IL-1β and MMP-3 levels in synovial fluid were detected. Results: Compared with OA model blank control group, OA model positive control group and OA model TSA low, medium and high dose group had significant difference (P <0.05). There was significant difference between OA model positive control group and OA model TSA high dose group (P <0.01). Compared with synovial HE staining and synovitis score, a lot of synovial tissue inflammatory cells Infiltration, fibrous tissue proliferation and synovial cell hyperplasia, disordered, more visible capillary synovial hyperplasia, showed severe synovitis performance, OA model positive control group, moderate synovial tissue inflammatory cell infiltration, fibrous tissue proliferation And synovial cell proliferation, irregular arrangement, a small amount of capillary proliferation around the synovial tissue showed moderate synovitis, OA model TSA high dose group of mild synovial inflammatory cell infiltration, fibrosis and synovial membrane Cell proliferation, arrangement rules, synovial tissue around the visible micro capillary proliferation, showed mild synovitis; β-catenin protein immunohistochemistry, OA model positive control group and OA model TSA high-dose group and OA model empty (P <0.01). There was significant difference between OA model positive control group and OA model TSA high dose group (P <0.05). Compared with normal group, the levels of TNF-1 and IL- (P <0.05). There was no significant difference between OA model high dose TSA group and OA model positive control group (P> 0.05), the difference between the two groups and OA model blank group There was statistical significance (P <0.01). Conclusion: Achyranthes saponin can effectively inhibit the synovial inflammation of OA, improve the degree of joint adhesion, decrease the expression of β-catenin in synovial tissue and decrease the content of TNF-1, IL-1β, MMP-3 in synovial fluid Explore further.