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目的:探讨药物中异烟肼的分光光度测定法。方法本文依据异烟肼分子中酰肼基的还原性,提出异烟肼将Cu2+氧化产生Cu+,Cu+与新铜试剂形成了一种黄色配合物,提出一种药物中异烟肼测定的新方法,即分光光度测定法。结果:当pH在0.4~0.8内,异烟肼浓度C在0~112.5μg/25mL以内服从比耳定律,测定的表观摩尔吸光系数ε为2.91×104L.mol-1.cm-1;当pH在0.4~0.8内,体系吸光度最大而且平坦;体系产物的测试波长为450nm;当体系中Cu2+溶液用量大于1.25mL时,体系吸光度最大且恒定。当体系中新铜试剂用量大于1.5 mL时,体系吸光度最大且恒定。在药物中异烟肼的测定里,所拟方法的测定结果明确,与KBrO3滴定法的结果一致。结论:使用分光光度法对药物中异烟肼进行测定,其操作简便、快捷,可以用于药厂其它药物的控制和分析。
Objective: To investigate the spectrophotometric method of isoniazid in medicine. Methods Based on the reducibility of the hydrazide groups of isoniazid molecules, it was proposed that isoniazid oxidized Cu 2+ to produce Cu +, which formed a yellow complex with a new copper reagent. A new method for the determination of isoniazid in pharmaceuticals was proposed , Ie spectrophotometry. Results: Beer’s law was obeyed when the isoniazid concentration C was in the range of 0-112.5 μg / 25 mL at a pH of 0.4-0.8. The apparent molar absorptivity ε was 2.91 × 104 L · mol-1.cm-1. The absorbance of the system is the largest and flat at the pH range of 0.4-0.8. The wavelength of the product of the system is 450 nm. When the amount of Cu2 + solution in the system is more than 1.25 mL, the absorbance of the system is the largest and the constant. When the amount of new copper reagent in the system is more than 1.5 mL, the absorbance of the system is the largest and constant. Determination of isoniazid in the drug, the determination of the proposed method is clear, consistent with the results of KBrO3 titration. Conclusion: The determination of isoniazid in pharmaceuticals by spectrophotometry is simple, rapid and can be used for the control and analysis of other pharmaceuticals.