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筛选能显著诱导马铃薯块茎抗晚疫病的微生物源激发子,并探讨其诱导抗病性机理。分别将31种微生物的发酵液(F-0)以及其中真菌和放线菌的胞内提取物(F-1)、胞壁提取物(F-2)单独或复配后诱导处理马铃薯块茎切片,对其中诱导抗病效果显著的激发子进一步分析其中的有效诱抗物质及诱导处理后的生理变化。获得了9种较好的激发子,诱抗效果均达50%以上,其中放线菌A5295发酵液型激发子诱抗效果最高,达63.97%,且单一激发子复配后诱抗活性更高,其中MK与A5295的复配型激发子诱抗效果最好,达66.67%。MK和A32910b发酵液中有效诱抗物质均为饱和硫酸铵沉淀(A组分)、以及硫酸铵沉淀后再用乙醇沉淀(B组分)所得的物质。诱导处理后块茎中POD、PAL、PPO活性均明显高于对照,且在抗性表现出之前迅速增加达到峰值。此外,块茎中可溶性蛋白含量比对照增加41.53%,并有一些新蛋白产生。结果表明复配激发子比单一激发子诱抗活性更高,激发子中有效诱抗物质均为A和B组分,诱导的抗病性可能与块茎中POD、PAL、PPO活性增高,可溶性蛋白含量增加以及新蛋白质的产生有关。
Screening for microbial source elicitors that can significantly induce potato tuberous resistance to late blight and to explore its mechanism of disease resistance. The potato tuber slices were induced after the fermentation broth (F-0) of 31 microorganisms and the intracellular extracts (F-1) and cell wall extracts (F-2) of fungi and actinomycetes alone or in combination , Which induced significant resistance effect of the elicitors further analysis of the effective inducing substances and induced physiological changes after treatment. Nine kinds of better excitons were obtained, and the inducing effect reached more than 50%. Actinomycete strain A5295 had the highest inducing effect of the elicitor of the fermentation liquid, reaching 63.97%, and the activity of the single elicitor was higher after compounding , Of which MK and A5295 had the best inductive effect with 66.67%. The effective inducing substances in MK and A32910b fermentation broth were saturated ammonium sulfate precipitation (A component) and precipitation with ammonium sulfate (B component) after ammonium sulfate precipitation. The activities of POD, PAL and PPO in the tubers after induction treatment were significantly higher than those of the control, and rapidly increased and reached the peak before the resistance showed. In addition, tuber soluble protein content increased 41.53% compared with the control, and some new protein production. The results showed that the complexed exciton induced more active than the single exciton, the effective inducers in the exciton were A and B components, and the induced disease resistance may be related to tuber higher POD, PAL, PPO activity, soluble protein Increased content and the production of new proteins.