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[目的]探讨加味左金丸对胃癌前病变大鼠胃黏膜组织细胞增殖与凋亡的影响。[方法]将以饮用甲硝基亚硝基胍为主24周制成的胃癌前病变模型大鼠随机分为模型组,维甲酸组,加味左金丸高、中、低剂量组,治疗16周,采用免疫组化SP法,TUNEL法检测胃黏膜组织细胞增殖细胞核抗原(PCNA)、Bcl-2蛋白及凋亡指数(AI)的表达情况。[结果]加味左金丸能明显下调胃癌前病变组织细胞的PCNA、Bcl-2的表达,上调AI。[结论]抑制细胞增殖,诱导细胞凋亡可能是该方的作用机制之一。
[Objective] To explore the effect of Jiawei Zuojin Pills on the proliferation and apoptosis of gastric mucosa in gastric precancerous rats. [Methods] Rats with gastric precancerous lesions made mainly of drinking nitro-nitrosoguanidine for 24 weeks were randomly divided into model group, retinoid group, and high-, middle-, and low-dose Zuojin Pills of Jiawei. The treatment was performed for 16 weeks. Immunohistochemical SP method and TUNEL method were used to detect the expression of proliferating cell nuclear antigen (PCNA), Bcl-2 protein and apoptosis index (AI) in gastric mucosa. [Result] Modified Zuojin Pill could obviously down-regulate the expression of PCNA and Bcl-2 in precancerous lesions and up-regulate AI. [Conclusion] The inhibition of cell proliferation and the induction of apoptosis may be one of the mechanisms of action of this side.