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目的:探讨矾冰纳米乳对L-929实验细胞增殖抑制作用。方法:采用细胞病变抑制法与MTT法,将试验药物稀释后加入培养4h贴壁的L-929细胞中,37℃、5%CO2培养箱中观察24h、48h,同时设传统药物矾冰液和正常细胞对照,倒置显微镜下观察药物对细胞形态的影响,测定OD值比较药物对细胞生长的影响。结果:矾冰纳米乳对L-929细胞的生长有随浓度增加其生长抑制率也增加的趋势,与矾冰液比较有统计学差异(P<0.05);矾冰纳米乳在同一浓度、同一时间内细胞增长抑制率低于矾冰液,差异有统计学意义(P<0.05);同时矾冰纳米乳有随浓度降低,细胞培养时间延长其细胞抑制率也降低,与正常组细胞比较无统计学差异(P>0.05);倒置显微镜下观察细胞形态良好。结论:纳米矾冰乳对L-929培养细胞无毒性作用,可以用于临床。
Objective: To investigate the inhibitory effect of alum ice nanoemulsion on the proliferation of L-929 cells. Methods: Cytopathic inhibitory method and MTT method were used. The test drugs were diluted and added to the adherent L-929 cells cultured for 4 hours. The cells were observed in 37 ℃ and 5% CO2 incubators for 24h and 48h. The normal cell control, under inverted microscope to observe the impact of drugs on cell morphology, OD values were measured to compare the impact of drugs on cell growth. Results: The alum ice nanoemulsion showed a tendency of increasing with increasing concentration of L-929 cells, and its growth inhibition rate also increased. Compared with alum ice fluid, there was a significant difference (P <0.05) The inhibitory rate of cell growth in time was lower than that of alum ice fluid, the difference was statistically significant (P <0.05); at the same time, the alum ice nanoemulsion decreased with concentration, and the cell inhibition rate was also decreased when the cell culture time prolonged, Statistical difference (P> 0.05); Under inverted microscope, the cell morphology was good. Conclusion: Nano-alum ice milk has no toxic effect on cultured L-929 cells and can be used clinically.