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目的建立熊胆川贝枇杷膏的质量标准。方法采用薄层色谱法鉴别熊胆粉、枇杷叶和薄荷脑药材,并用HPLC法测定制剂中的牛磺熊去氧胆酸及岩白菜素的含量。结果薄层色谱特征斑点清晰,阴性样品无干扰。0.05~0.73 mg·m L-1牛磺熊去氧胆酸、7.7~154μg·m L-1岩白菜素与峰面积呈良好的线性关系(r=0.9999),平均回收率分别为98.0%、99.2%,RSD分别为0.75%、0.65%。结论所用方法简便、准确、专属性好,可作为熊胆川贝枇杷膏的质量控制方法。
Objective To establish the quality standard of Xiongchuan Chuanbei Loquat Paste. Methods Thin layer chromatography was used to identify the powder of bear bile powder, loquat leaf and menthol. The contents of tauroursodeoxycholic acid and bergenin in the preparations were determined by HPLC. Results TLC characteristics of spots clear, negative samples without interference. 0.05 ~ 0.73 mg · m L-1 tauroursodeoxycholic acid and 7.7 ~ 154 μg · m L-1 of bergenin showed a good linear relationship with peak area (r = 0.9999). The average recoveries were 98.0% 99.2%, RSD respectively 0.75%, 0.65%. Conclusion The method used is simple, accurate and specific and can be used as a quality control method for Xiongchuan Chuanbei Loquat Paste.