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背景研究证明荞麦种子有降糖、降脂及抗氧化作用,而有关荞麦叶对脂蛋白失调的影响的研究尚较缺乏。目的研究荞麦叶总黄酮(totalFlavonesofbuckwheatleaf,TFBL)对血脂及脂质过氧化物的影响。设计完全随机对照的实验研究。地点、材料和干预实验在华北煤炭医学院药理学教研室和华北煤炭医学院附属医院同位素科完成。实验选用SD大鼠30只,清洁级,雌雄各半。用高脂饲料诱发大鼠高脂血症,口服TFBL0.3g/(kg·d),干预治疗12周。主要观察指标①TFBL对高脂血症大鼠血脂的影响。②TFBL对高脂血症大鼠血清及肝组织丙二醛和超氧化物歧化酶(superoxidasedismu-tase,SOD)的影响。结果TFBL组高脂血症大鼠血清三酰甘油、载脂蛋白B和低密度脂蛋白胆固醇[(0.49±0.11),(0.04±0.02),(1.78±0.31)mmol/L]明显低于模型组[(0.73±0.15),(0.06±0.02),(2.47±0.13)mmol/L](t=4.080,P<0.01;t=2.401,P<0.05;t=6.491,P<0.01)。TFBL组血和肝组织丙二醛也明显低于模型组(t=3.888,2.667,P<0.05)。同时TFBL还能明显增加血中SOD活力(t=2.765,P<0.05)。结论TFBL具有调血脂和抗脂质过氧化作用。
Background studies have shown that buckwheat seeds have hypoglycemic, lipid-lowering and anti-oxidant effects, while studies on the effects of buckwheat leaves on lipoprotein imbalances are still lacking. Objective To study the effect of total Flavones of Buckwheat Leaf Extract (TFBL) on blood lipids and lipid peroxidation. Design completely randomized controlled experimental studies. Locations, materials, and intervention experiments were performed in the Department of Pharmacology, North China Coal Medical College and the Isotope Division of the Affiliated Hospital of North China Coal Medical College. The experiment used 30 SD rats, clean grade, male and female. Hyperlipidemia was induced in rats with high-fat diet. Oral TFBL 0.3g/(kg·d) was administered for 12 weeks. MAIN OUTCOME MEASURES 1 Effect of TFBL on blood lipids in hyperlipidemic rats. Effects of 2TFBL on malondialdehyde and superoxide dismutase (SOD) in serum and liver tissue of hyperlipidemic rats. Results Serum triacylglycerol, apolipoprotein B, and low density lipoprotein cholesterol in the TFBL group were significantly lower than those in the model [(0.49±0.11), (0.04±0.02), (1.78±0.31) mmol/L]. The groups [(0.73±0.15), (0.06±0.02), (2.47±0.13) mmol/L] (t=4.080, P<0.01; t=2.401, P<0.05; t=6.491, P<0.01). Malondialdehyde in blood and liver tissue was also significantly lower in the TFBL group than in the model group (t=3.888, 2.667, P<0.05). At the same time, TFBL also significantly increased the SOD activity in blood (t=2.765, P<0.05). Conclusion TFBL has lipid-regulating and anti-lipid peroxidation effects.