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目的 研究耐多药结核分枝杆菌katG、inhA基因的变异机理。方法 用katG、inhA基因片段为引物 ,聚合酶链反应扩增产物 ,克隆后制备质粒 ,直接测定DNA序列。结果 15株耐异烟肼菌株均有katG基因突变 ,主要为点突变 ,其中 14株有 4 63位和 3 15位AA密码子改变。在 18个耐异烟肼菌株中 ,全部出现基因inhA基因变异 ,主要为点突变和缺失 ,以单个位点变异为主 ,各菌株变异不同。katG、inhA基因同时出现变异的比例高。结论 结核分枝杆菌耐药基因变异复杂 ,我国耐多药结核菌株与国外的菌株katG、inhA基因变异特点不同。
Objective To study the variation mechanism of multidrug-resistant Mycobacterium tuberculosis katG and inhA genes. Methods The katG and inhA gene fragments were used as primers to amplify the products by polymerase chain reaction. After cloning, plasmids were prepared and DNA sequences were directly determined. Results All of the 15 isoniazid-resistant isolates had katG gene mutations, mainly point mutations. Among them, 14 strains had 4 63 and 3 15 AA codon changes. Among the 18 isoniazid-resistant isolates, all the inhA gene mutations were found, which were mainly point mutation and deletion. katG, inhA gene mutation at the same time a high percentage. Conclusion The variation of drug resistance genes in Mycobacterium tuberculosis is complex. The characteristics of katG and inhA genes in MDR-TB strains in China are different from those in other countries.