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目的观察HBV慢性感染携带者和慢性乙型肝炎患者的血小板参数及CD62P的变化规律。方法采用流式细胞术(FCM)测定HBV携带者、慢性乙型肝炎患者外周血中血小板膜糖蛋白CD62P的阳性表达率,同时对其血小板参数进行检测,并与55例健康对照组进行比较分析。结果 HBV携带者、慢性乙型肝炎患者体内血小板活化标志物CD62P的表达率分别为(4.00±0.93)%、(15.36±6.32)%,较健康对照组的(1.40±0.55)%显著升高,差异均有统计学意义(P<0.01),且慢性乙型肝炎患者体内血小板活化水平较HBV携带者显著升高(P<0.01);各组组间比较差异有统计学意义(F=389.6,P<0.01)。HBV携带者、慢性乙型肝炎患者血小板(PLT)均低于健康对照组(P<0.05),平均血小板体积(MPV)均高于健康对照组(P<0.05),HBV携带者、慢性乙型肝炎患者间PLT、MPV差异无统计学意义(P>0.05);各组间血小板比容(PCT)、血小板体积分布宽度(PDW)和大血小板比率(P-LCR)差异无统计学意义(P>0.05)。结论高表达的CD62P可能参与慢性乙型肝炎的发生和发展,联合PLT与MPV的测定可作为病毒性肝炎疾病进展及疗效观察的指标。
Objective To observe the changes of platelet parameters and CD62P in carriers of chronic HBV infection and chronic hepatitis B patients. Methods Flow cytometry (FCM) was used to detect the positive expression rate of CD62P in peripheral blood of HBV carriers and chronic hepatitis B patients. Meanwhile, the platelet parameters were measured and compared with 55 healthy controls . Results The positive rates of CD62P in HBV carriers and chronic hepatitis B patients were (4.00 ± 0.93)% and (15.36 ± 6.32)%, respectively, which were significantly higher than those in healthy controls (1.40 ± 0.55% (P <0.01), and the level of platelet activation in patients with chronic hepatitis B was significantly higher than that in HBV carriers (P <0.01). There was significant difference between the two groups (F = 389.6, P <0.01). (P <0.05), and mean platelet volume (MPV) in patients with HBV carriers and chronic hepatitis B were significantly lower than those in healthy controls (P <0.05). HBV carriers, chronic type B There was no significant difference in PLT and MPV among patients with hepatitis (P> 0.05). There was no significant difference in PCT, PDW and P-LCR between groups (P > 0.05). Conclusion High expression of CD62P may be involved in the occurrence and development of chronic hepatitis B. The combination of PLT and MPV can be used as an indicator of the progress of disease and the curative effect of viral hepatitis.