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目的 了解幽门螺杆菌(Helicobacter pylori, Hp) 对甲硝唑的耐药状况, 研究相关的多重基因突变, 为深入揭示Hp耐药的分子机制提供相关资料。方法 分离培养54株Hp, 以琼脂稀释法检测Hp的甲硝唑最低抑菌浓度(deterimination of minimal inhibitory concentration, MIC), 用PCR方法扩增甲硝唑耐药相关基因(rdxA、frxA、fdxB), 经分子克隆后DNA测序, 分析敏感株和耐药株耐药基因的序列及其与耐药性的关系。结果 武汉地区人群Hp对甲硝唑的耐药率为67%; 甲硝唑耐药基因序列分析表明, 耐药临床株的rdxA和(或) frxA基因存在插入、缺失和点突变。结论 Hp对甲硝唑耐药不仅与rdxA有关, 同时存在frxA、fdxB基因突变。frxA、fdxB基因的突变可能与rdxA在Hp耐甲硝唑中有协同作用。
Objective To understand the drug resistance of Helicobacter pylori (Hp) to metronidazole and to study the multiple gene mutations associated with it, and to provide relevant information for further revealing the molecular mechanism of Hp resistance. Methods 54 strains of Hp were isolated and cultured, the MICs of metronidazole were determined by agar dilution method, and the metronidazole resistance-related genes (rdxA, frxA, fdxB) were amplified by PCR. , The molecular cloning of DNA sequencing, analysis of sensitive and resistant strains resistant gene sequence and its relationship with drug resistance. Results The resistance rate of Hp to metronidazole in Wuhan population was 67%. The sequence analysis of metronidazole resistance gene showed that the rdxA and / or frxA gene of the drug-resistant clinical strains had insertion, deletion and point mutation. Conclusion The resistance of metronidazole to Hp is not only related to rdxA, but also to the mutations of frxA and fdxB genes. Mutations of frxA and fdxB genes may have a synergistic effect with rdxA in Hp-resistant metronidazole.